暑假華藝文章8-台灣北區人類、家犬與雞中止蛋之綠膿桿菌抗藥性調查
文章名稱:台灣北區人類、家犬與雞中止蛋之綠膿桿菌抗藥性調查 Antimicrobial Resistance of Pseudomonas aeruginosa of Humans, Canines and Death of Chicken Embryos in Northern Taiwan
作者名稱:孫友梅 , 碩士 指導教授：張紹光
資料來源:無
內容摘要: 本實驗的目的為比較人類病患、伴侶動物與經濟動物所分離之綠膿桿菌在抗生素敏感性及數種抗藥性基因型的表現。試驗期間，由人類病患分得94株、家犬分得29株以及雞中止蛋分得97株，共220株綠膿桿菌；經確認之菌株分別進行抗生素敏感性試驗、脈衝式電泳及利用聚合酶鏈鎖反應偵測Ambler class A（blaTEM、blaSHV、blaGES、blaVEB、blaPER、blaPSE）與Ambler class D（blaOXA-2 like、blaOXA-10 like）之乙內醯胺酶。
抗生素敏感性試驗結果顯示，所選用之盤尼西林類、頭孢子素類、胺基醣苷類與氟奎諾酮類抗菌劑中，人類病患分離株之抗藥性皆最嚴重，家犬次之，中止蛋最低；對所偵測的抗藥性基因而言，TEM-1在人類、家犬、雞蛋分離株之陽性率分別為90.4%、69.0%與71.1%，PSE-1之陽性率分別77.7%、75.9%與6.2%，OXA-10 like之陽性率則為98.9%、86.2%與18.6%，而乙內醯胺酶SHV、GES、VEB、PER及OXA-2 like皆未在本實驗中被偵測出。經定序後，發現OXA-17及OXA-10...
The aims of the study were to survey and differentiate the phenotype and genotype of Pseudomonas aeruginosa from human, companion and economic animal isolates. A total of ninety four human isolates, twenty nine canines isolates and ninety seven chicken isolates used polymerase chain reaction (PCR) to detect the encoding genes of class A (blaTEM, blaSHV, blaGES, blaVEB, blaPER and blaPSE) and class D (blaOXA-2 like, and blaOXA-10 like) β-lactamases. All isolates were also performed antimicrobial susceptibility testing and pulsed-fielded gel electrophoresis (PFGE).
Antimicrobial resistance of human isolates were higher than canine isolates, followed by chicken isolates. blaTEM, blaSHV, blaGES, blaVEB, blaPER ,and blaOXA-2 like were not harboured in any isolates. In human, canine and chicken isolates, blaTEM-1 (90.4%, 69.0% and 71.1%), blaPSE-1 (77.7%, 75.9% and 6.2%) and blaOXA-10 like (98.9%, 86.2% and 18.6%) were detected, respectively. OXA-17 was the most common OXA enzyme in human and canine isolates, followed by OXA-10. However, the results of the prevalence of OXA-10 and OXA...
參考文獻:
1.116. Tipper DJ, Strominger JL. Mechanism of action of penicillins: a proposal based on their structural similarity to acyl-D-alanyl-D-alanine. Proc Natl Acad Sci U S A. 54: 1133-41, 1965.
連結：
2.117. Thomson KS, Sanders CC. Detection of extended-spectrum beta-lactamases in members of the family Enterobacteriaceae: comparison of the double-disk and three-dimensional tests. Antimicrob Agents Chemother. 36: 1877-82, 1992.
連結：
3.118. Toleman MA, Vinodh H, Sekar U, Kamat V, Walsh TR. blaVIM-2-harboring integrons isolated in India, Russia, and the United States arise from an ancestral class 1 integron predating the formation of the 3' conserved sequence. Antimicrob Agents Chemother. 51: 2636-8, 2007.
連結：
4.119. Tolar EL, Hendrix DV, Rohrbach BW, Plummer CE, Brooks DE, Gelatt KN. Evaluation of clinical characteristics and bacterial isolates in dogs with bacterial keratitis: 97 cases (1993-2003). J Am Vet Med Assoc. 228: 80-5, 2006.
連結：
5.120. Unal S, Garcia-Rodriguez JA. Activity of meropenem and comparators against Pseudomonas aeruginosa and Acinetobacter spp. isolated in the MYSTIC Program, 2002-2004. Diagn Microbiol Infect Dis. 53: 265-71, 2005.
作者名稱:孫友梅 , 碩士 指導教授：張紹光
資料來源:無
內容摘要: 本實驗的目的為比較人類病患、伴侶動物與經濟動物所分離之綠膿桿菌在抗生素敏感性及數種抗藥性基因型的表現。試驗期間，由人類病患分得94株、家犬分得29株以及雞中止蛋分得97株，共220株綠膿桿菌；經確認之菌株分別進行抗生素敏感性試驗、脈衝式電泳及利用聚合酶鏈鎖反應偵測Ambler class A（blaTEM、blaSHV、blaGES、blaVEB、blaPER、blaPSE）與Ambler class D（blaOXA-2 like、blaOXA-10 like）之乙內醯胺酶。
抗生素敏感性試驗結果顯示，所選用之盤尼西林類、頭孢子素類、胺基醣苷類與氟奎諾酮類抗菌劑中，人類病患分離株之抗藥性皆最嚴重，家犬次之，中止蛋最低；對所偵測的抗藥性基因而言，TEM-1在人類、家犬、雞蛋分離株之陽性率分別為90.4%、69.0%與71.1%，PSE-1之陽性率分別77.7%、75.9%與6.2%，OXA-10 like之陽性率則為98.9%、86.2%與18.6%，而乙內醯胺酶SHV、GES、VEB、PER及OXA-2 like皆未在本實驗中被偵測出。經定序後，發現OXA-17及OXA-10...
The aims of the study were to survey and differentiate the phenotype and genotype of Pseudomonas aeruginosa from human, companion and economic animal isolates. A total of ninety four human isolates, twenty nine canines isolates and ninety seven chicken isolates used polymerase chain reaction (PCR) to detect the encoding genes of class A (blaTEM, blaSHV, blaGES, blaVEB, blaPER and blaPSE) and class D (blaOXA-2 like, and blaOXA-10 like) β-lactamases. All isolates were also performed antimicrobial susceptibility testing and pulsed-fielded gel electrophoresis (PFGE).
Antimicrobial resistance of human isolates were higher than canine isolates, followed by chicken isolates. blaTEM, blaSHV, blaGES, blaVEB, blaPER ,and blaOXA-2 like were not harboured in any isolates. In human, canine and chicken isolates, blaTEM-1 (90.4%, 69.0% and 71.1%), blaPSE-1 (77.7%, 75.9% and 6.2%) and blaOXA-10 like (98.9%, 86.2% and 18.6%) were detected, respectively. OXA-17 was the most common OXA enzyme in human and canine isolates, followed by OXA-10. However, the results of the prevalence of OXA-10 and OXA...
參考文獻:
1.116. Tipper DJ, Strominger JL. Mechanism of action of penicillins: a proposal based on their structural similarity to acyl-D-alanyl-D-alanine. Proc Natl Acad Sci U S A. 54: 1133-41, 1965.
連結：
2.117. Thomson KS, Sanders CC. Detection of extended-spectrum beta-lactamases in members of the family Enterobacteriaceae: comparison of the double-disk and three-dimensional tests. Antimicrob Agents Chemother. 36: 1877-82, 1992.
連結：
3.118. Toleman MA, Vinodh H, Sekar U, Kamat V, Walsh TR. blaVIM-2-harboring integrons isolated in India, Russia, and the United States arise from an ancestral class 1 integron predating the formation of the 3' conserved sequence. Antimicrob Agents Chemother. 51: 2636-8, 2007.
連結：
4.119. Tolar EL, Hendrix DV, Rohrbach BW, Plummer CE, Brooks DE, Gelatt KN. Evaluation of clinical characteristics and bacterial isolates in dogs with bacterial keratitis: 97 cases (1993-2003). J Am Vet Med Assoc. 228: 80-5, 2006.
連結：
5.120. Unal S, Garcia-Rodriguez JA. Activity of meropenem and comparators against Pseudomonas aeruginosa and Acinetobacter spp. isolated in the MYSTIC Program, 2002-2004. Diagn Microbiol Infect Dis. 53: 265-71, 2005.
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