暑假華藝文章10-台灣豬瘟病毒分子流行病學之研究
文章名稱:台灣豬瘟病毒分子流行病學之研究 Molecular Epidemiological Studies of Classical Swine Fever Viruses in Taiwan
作者名稱:潘居祥 , 博士 指導教授：賴秀穗
資料來源:無
內容摘要:豬瘟是由豬瘟病毒引起之高接觸性傳染病，親緣樹分析有助於追蹤豬瘟病毒發生的地理起源並了解病毒基因型別的分佈。將1989至2007年台灣分離到的167株豬瘟病毒，針對Erns及E2封套醣蛋白基因以RT-PCR增幅此兩區間並進行核酸定序及親緣樹分析。雖然兩者出現類似的樹形圖，但Erns比E2具有更好的區別效果。親緣樹分析結果顯示，124個田間分離株屬於2.1及2.2亞群，此兩亞群都屬於外來型病毒株，其餘的43個田間分離株則屬於3.4亞群的本土型病毒株。由於2.1亞群病毒株在Erns親緣樹分析時可進一步區分成信賴值(bootstrap values)高達98%及85%的兩個群組(clusters)，顯示此兩群組間病毒的核酸序列有明顯差異，因此我們認為2.1亞群應再進一步區分為2.1a及2.1b。其中2.1a 亞群病毒株最早於1994年入侵台灣，1995年即爆發流行，之後成為田間優勢族群至今。然而3.4亞群病毒株盛行於1994年以前，但自1996年以後就無法從田間分離到此型病毒。過去近二十年間，我們看到台灣田間流行的豬瘟病毒從3.4亞群轉變成2.1a 亞群，這基因型轉變並非由本土型病毒株的基因突...
Classical swine fever (CSF) is a highly contagious viral disease of swine caused by classical swine fever virus (CSFV). Phylogenetic analysis of CSFV field isolates are useful to trace the geographic origins of the disease and to understand the distribution of CSFV genotypes. Two envelope glycoprotein (Erns and E2) regions of CSFV were amplified by reverse transcription-polymerase chain reaction (RT-PCR) and sequenced directly from 167 specimens collected between 1989 and 2007 in Taiwan. Phylogenetic analysis of the two regions revealed a similar tree topology and, furthermore, the Erns region provided better discrimination of CSFV genotypes than the E2 region. Of the 167 isolates collected, 124 were clustered within subgroups 2.1 and 2.2, which were considered to be potential exotic strains, whereas the remaining 43 isolates were clustered within subgroup 3.4, which is considered to contain the historical strains. Since the subgroup 2.1 could be further separated into two different clusters with high bootstrap values of 98% and 85% in the Erns tree, we proposed that subgroup 2.1 sho...
參考文獻:1.Barlic-Maganja D, Grom J, 2001. Highly sensitive one-tube RT-PCR and microplate hybridisation assay for the detection and for the discrimination of classical swine fever virus from other pestiviruses. J Virol Methods 95:101-110.
連結：
2.Bartak P, Greiser-Wilke I, 2000. Genetic typing of classical swine fever virus isolates from the territory of the Czech Republic. Vet Microbiol 77: 59-70.
連結：
3.Baxi MK, Baxi S, Clavijo A, Burton KM, Deregt D, 2006. Microarray-based detection and typing of foot-and-mouth disease virus. Vet J 172:473-481.
連結：
4.Biagetti M, Greiser-Wilke I, Rutili D, 2001. Molecular epidemiology of classical swine fever in Italy. Vet Microbiol 83: 205-215.
連結：
5.Bjorklund HV, Stadejek T, Vilcek S, Belak S, 1998. Molecular characterization of the 3' noncoding region of classical swine fever virus vaccine strains. Virus Genes 16:307-312.
作者名稱:潘居祥 , 博士 指導教授：賴秀穗
資料來源:無
內容摘要:豬瘟是由豬瘟病毒引起之高接觸性傳染病，親緣樹分析有助於追蹤豬瘟病毒發生的地理起源並了解病毒基因型別的分佈。將1989至2007年台灣分離到的167株豬瘟病毒，針對Erns及E2封套醣蛋白基因以RT-PCR增幅此兩區間並進行核酸定序及親緣樹分析。雖然兩者出現類似的樹形圖，但Erns比E2具有更好的區別效果。親緣樹分析結果顯示，124個田間分離株屬於2.1及2.2亞群，此兩亞群都屬於外來型病毒株，其餘的43個田間分離株則屬於3.4亞群的本土型病毒株。由於2.1亞群病毒株在Erns親緣樹分析時可進一步區分成信賴值(bootstrap values)高達98%及85%的兩個群組(clusters)，顯示此兩群組間病毒的核酸序列有明顯差異，因此我們認為2.1亞群應再進一步區分為2.1a及2.1b。其中2.1a 亞群病毒株最早於1994年入侵台灣，1995年即爆發流行，之後成為田間優勢族群至今。然而3.4亞群病毒株盛行於1994年以前，但自1996年以後就無法從田間分離到此型病毒。過去近二十年間，我們看到台灣田間流行的豬瘟病毒從3.4亞群轉變成2.1a 亞群，這基因型轉變並非由本土型病毒株的基因突...
Classical swine fever (CSF) is a highly contagious viral disease of swine caused by classical swine fever virus (CSFV). Phylogenetic analysis of CSFV field isolates are useful to trace the geographic origins of the disease and to understand the distribution of CSFV genotypes. Two envelope glycoprotein (Erns and E2) regions of CSFV were amplified by reverse transcription-polymerase chain reaction (RT-PCR) and sequenced directly from 167 specimens collected between 1989 and 2007 in Taiwan. Phylogenetic analysis of the two regions revealed a similar tree topology and, furthermore, the Erns region provided better discrimination of CSFV genotypes than the E2 region. Of the 167 isolates collected, 124 were clustered within subgroups 2.1 and 2.2, which were considered to be potential exotic strains, whereas the remaining 43 isolates were clustered within subgroup 3.4, which is considered to contain the historical strains. Since the subgroup 2.1 could be further separated into two different clusters with high bootstrap values of 98% and 85% in the Erns tree, we proposed that subgroup 2.1 sho...
參考文獻:1.Barlic-Maganja D, Grom J, 2001. Highly sensitive one-tube RT-PCR and microplate hybridisation assay for the detection and for the discrimination of classical swine fever virus from other pestiviruses. J Virol Methods 95:101-110.
連結：
2.Bartak P, Greiser-Wilke I, 2000. Genetic typing of classical swine fever virus isolates from the territory of the Czech Republic. Vet Microbiol 77: 59-70.
連結：
3.Baxi MK, Baxi S, Clavijo A, Burton KM, Deregt D, 2006. Microarray-based detection and typing of foot-and-mouth disease virus. Vet J 172:473-481.
連結：
4.Biagetti M, Greiser-Wilke I, Rutili D, 2001. Molecular epidemiology of classical swine fever in Italy. Vet Microbiol 83: 205-215.
連結：
5.Bjorklund HV, Stadejek T, Vilcek S, Belak S, 1998. Molecular characterization of the 3' noncoding region of classical swine fever virus vaccine strains. Virus Genes 16:307-312.
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