護理科 陳柏文老師的歷程檔案

專題研究計畫名稱	2012乳鐵蛋白耐受性益生菌之促進宿主功效分析
資料日期	2012-01-01

[摘要] :
益生菌已被證實具有許多生理功能，其可以活化免疫系統、維持腸道菌相平衡、預防抗生素引起的下痢、抑制多種病原菌的入侵以及抑制腫瘤細胞生長等功效。我們目前已獲得數株能被牛乳鐵蛋白（lactoferrin from bovine）所促進生長之益生菌菌株，因此牛乳鐵蛋白也能當做益生素使用。本研究將進一步研究人乳鐵蛋白（lactoferrin from human）是否也能促進這些益生菌菌株生長；另一方面，也會研究併用乳鐵蛋白與這些益生菌分泌物後之促進宿主免疫調節功效。研究將採用cytokine array方法（系統性方式）和酵素連結免疫分析法（ELISA）去探討益生菌分泌物與乳鐵蛋白併用後，對巨嗜細胞與數突狀細胞分泌細胞激素之影響，藉由細胞素之表現改變情形，去探討乳鐵蛋白與益生菌併用後之調節免疫活性功效，最後也將研究這些影響的機轉為何；另外，我們也將一併研究併用乳鐵蛋白與益生菌後之抑制有害菌生長之功效與其可能機轉。

[參考文獻] :
de Vrese, M. and J. Schrezenmeir, Probiotics, prebiotics, and synbiotics. Adv Biochem Eng Biotechnol, 2008. 111: p. 1-66.
2. Gaggia, F., P. Mattarelli, and B. Biavati, Probiotics and prebiotics in animal feeding for safe food production. Int J Food Microbiol, 2010. 141 Suppl 1: p. S15-28.
3. Gupta, V. and R. Garg, Probiotics. Indian J Med Microbiol, 2009. 27: p. 202-9.
4. Vasiljevic, T. and N.P. Shah, Probiotics—From Metchnikoff to bioactives. Int Dairy J, 2008. 18: p. 714-28.
5. Oelschlaeger, T.A., Mechanisms of probiotic actions - A review. Int J Med Microbiol, 2010. 300(1): p. 57-62.
6. Shah, N.P., Probiotics and prebiotics. Agro-Food Industry Hi-tech, 2004. 15: p. 13-6.
7. Galdeano, C.M., et al., Proposed model: mechanisms of immunomodulation induced by probiotic bacteria. Clin Vaccine Immunol, 2007. 14(5): p. 485-92.
8. Gill, H.S., et al., Enhancement of immunity in the elderly by dietary supplementation with the probiotic Bifidobacterium lactis HN019. Am J Clin Nutr, 2001. 74(6): p. 833-9.
9. Wagner, E., C. Keusch, and C. Iben, Influence of the feed base excess on urine parameters in cats. J Anim Physiol Anim Nutr (Berl), 2006. 90(1-2): p. 19-24.
10. Kitazawa, H., et al., A novel immunostimulating aspect of Lactobacillus gasseri: induction of “Gasserokine” as chemoattractants for macrophages. . Int J Food Microbiol, 2002. 25: p. 29-38.
11. Moineau, S. and J. Goulet, Effect of feeding fermented milks on the pulmonary macrophage activity in mice. Milchwiss, 1991. 46: p. 551-4.
12. Nagao, F., et al., Effects of a fermented milk drink containing Lactobacillus casei strain Shirota on the immune system in healthy human subjects. Biosci Biotechnol Biochem, 2000. 64(12): p. 2706-8.
13. Aattouri, N. and D. Lemonnier, Production of interferon induced by Streptococcus thermophilus: role of CD4+ and CD8+ lymphocytes. J Nutr Biochem, 1997. 8: p. 25-31.
14. De Vrese, M., et al., Antiallergenes Potenzial von Milchsäure-produzierenden Bakterien. der Agrar- und Ernährungswissenschaftlichen Fakultät der CAU Kiel 2006: p. 205-14.
15. de Vrese, M., et al., Probiotic bacteria stimulate virus-specific neutralizing antibodies following a booster polio vaccination. Eur J Nutr, 2005. 44(7): p. 406-13.
16. Fukushima, Y., et al., Effect of a probiotic formula on intestinal immunoglobulin. A production in healthy children. Int J Food Microbiol, 1998. 42: p. 39-44.
17. Kaila, M., et al., Enhancement of the circulating antibody secreting cell response in human diarrhea by a human Lactobacillus strain Pediatr Res, 1992. 32: p. 141-44.
18. Marteau, P., et al., Effects of intrajejunal perfusion and chronic ingestion of Lactobacillus johnsonii strain La1 on serum concentrations and jejunal secretions of immunoglobulins and serum proteins in healthy humans. Gastroenterol Clin Biol, 1997. 21(4): p. 293-8.
19. de Moreno de LeBlanc, A., C. Matar, and G. Perdigon, The application of probiotics in cancer. Br J Nutr, 2007. 98 Suppl 1: p. S105-10.
20. Kim, J.E., et al., Cancer chemopreventive effects of lactic acid bacteria. J Microbiol Biotechnol, 2007. 17(8): p. 1227-35.
21. Aso, Y. and H. Akazan, Prophylactic effect of a Lactobacillus casei preparation on the recurrence of superficial bladder cancer. BLP Study Group. Urol Int, 1992. 49(3): p. 125-9.
22. Aso, Y., et al., Preventive effect of a Lactobacillus casei preparation on the recurrence of superficial bladder cancer in a double-blind trial. The BLP Study Group. Eur Urol, 1995. 27(2): p. 104-9.
23. de Roos, N.M. and M.B. Katan, Effects of probiotic bacteria on diarrhea, lipid metabolism, and carcinogenesis: a review of papers published between 1988 and 1998. Am J Clin Nutr, 2000. 71(2): p. 405-11.
24. Friend, B.A., R.E. Farmer, and K.M. Shahani, Effect of feeding and intraperitoneal implantation of yoghurt culture cells on Ehrlich ascites tumor. Milchwiss, 1982. 37: p. 708-10.
25. Bolognani, F., et al., Effect of lactobacilli, bifidobacteria and inulin on the formation of aberrant crypt foci in rats. Eur J Nutr, 2001. 40(6): p. 293-300.
26. Balansky, R., et al., Inhibitory effects of freeze-dried milk fermented by selected Lactobacillus bulgaricus strains on carcinogenesis induced by 1,2-dimethylhydrazine in rats and by diethylnitrosamine in hamsters. Cancer Lett, 1999. 147(1-2): p. 125-37.
27. Fukui, M., et al., The tumor-preventing effect of a mixture of several lactic acid bacteria on 1,2-dimethylhydrazine-induced colon carcinogenesis in mice. Oncol Rep, 2001. 8(5): p. 1073-8.
28. Lee do, K., et al., Anti-proliferative effects of Bifidobacterium adolescentis SPM0212 extract on human colon cancer cell lines. BMC Cancer, 2008. 8: p. 310.
29. Sorensen, M. and S.P.L. Sorensen, The proteins in whey. Compt. Rendus Laboratoire Carlsberg, 1939. 23: p. 55-9.
30. Groves, M.L., The isolation of a red protein from milk. J Am Chem Soc, 1960. 82: p. 3345-50.
31. Johanson, B., Isolation of an iron-containing red protein from human milk. Acta Chem Scand, 1960. 14: p. 510-2.
32. Montreuil, J., J. Tonnelat, and S. Mullet, Preparation and prosperities of lactotransferrin of human milk. Biochim Biophys Acta 1960. 45: p. 413-21.
33. Masson, P.L., J.F. Heremans, and E. Schonne, Lactoferrin, an iron-binding protein in neutrophilic leukocytes. J Exp Med, 1969. 130(3): p. 643-58.
34. Miyauchi, J. and Y. Watanabe, Immunocytochemical localization of lactoferrin in human neutrophils. An ultrastructural and morphometrical study. Cell Tissue Res, 1987. 247(2): p. 249-58.
35. Esaguy, N., A.P. Aguas, and M.T. Silva, High-resolution localization of lactoferrin in human neutrophils: labeling of secondary granules and cell heterogeneity. J Leukoc Biol, 1989. 46(1): p. 51-62.
36. Butler, T.W., et al., Assessment of total immunoreactive lactoferrin in hematopoietic cells using flow cytometry. J Immunol Methods, 1988. 108(1-2): p. 159-70.
37. Baker, E.N., H.M. Baker, and R.D. Kidd, Lactoferrin and transferrin: functional variations on a common structural framework. Biochem Cell Biol, 2002. 80(1): p. 27-34.
38. Baker, E.N., et al., Three-dimensional structure of lactoferrin in various functional states. Adv Exp Med Biol, 1994. 357: p. 1-12.
39. Bullen, J.J., H.J. Rogers, and E. Griffiths, Role of iron in bacterial infection. Curr Top Microbiol Immunol, 1978. 80: p. 1-35.
40. Brock, J.H., Lactoferrin in human milk: its role in iron absorption and protection against enteric infection in the newborn infant. Arch Dis Child, 1980. 55(6): p. 417-21.
41. Finkelstein, R.A., C.V. Sciortino, and M.A. McIntosh, Role of iron in microbe-host interactions. Rev Infect Dis, 1983. 5 Suppl 4: p. S759-77.
42. Weinberg, E.D., Iron withholding: a defense against infection and neoplasia. Physiol Rev, 1984. 64(1): p. 65-102.
43. Nemet, K. and I. Simonovits, The biological role of lactoferrin. Haematologia (Budap), 1985. 18(1): p. 3-12.
44. Levay, P.F. and M. Viljoen, Lactoferrin: a general review. Haematologica, 1995. 80(3): p. 252-67.
45. Masson, P.L., J.F. Heremans, and C. Dive, An iron-binding protein common to many external secretions. . Clin Chem Acta, 1966. 14: p. 735-9.
46. Masson, P.L. and J.F. Heremans, Lactoferrin in milk from different species. Comp Biochem Physiol B, 1971. 39(1): p. 119-29.
47. Lonnerdal, B. and S. Iyer, Lactoferrin: molecular structure and biological function. Annu Rev Nutr, 1995. 15: p. 93-110.
48. Orsi, N., The antimicrobial activity of lactoferrin: current status and perspectives. Biometals, 2004. 17(3): p. 189-96.
49. Chierici, R., Antimicrobial actions of lactoferrin. Adv Nutr Res, 2001. 10: p. 247-69.
50. Jenssen, H. and R.E. Hancock, Antimicrobial properties of lactoferrin. Biochimie, 2009. 91(1): p. 19-29.
51. Paul-Eugene, N., et al., [Immunomodulatory and anti-oxidant effects of bovine lactoferrin in man]. C R Acad Sci III, 1993. 316(2): p. 113-9.
52. Ye, X.Y., et al., Ribonuclease, cell-free translation-inhibitory and superoxide radical scavenging activities of the iron-binding protein lactoferrin from bovine milk. Int J Biochem Cell Biol, 2000. 32(2): p. 235-41.
53. Trif, M., et al., Liposomes as possible carriers for lactoferrin in the local treatment of inflammatory diseases. Exp Biol Med (Maywood), 2001. 226(6): p. 559-64.
54. Legrand, D., et al., Lactoferrin and host defence: an overview of its immuno-modulating and anti-inflammatory properties. Biometals, 2004. 17(3): p. 225-9.
55. Baveye, S., et al., Lactoferrin: a multifunctional glycoprotein involved in the modulation of the inflammatory process. Clin Chem Lab Med, 1999. 37(3): p. 281-6.
56. Tomita, M., et al., Twenty-five years of research on bovine lactoferrin applications. Biochimie, 2009. 91(1): p. 52-7.
57. Hyronimus, B., et al., Acid and bile tolerance of spore-forming lactic acid bacteria. Int J Food Microbiol, 2000. 61(2-3): p. 193-7.
58. Yokota, A., et al., Cholate resistance in Lactococcus lactis is mediated by an ATP-dependent multispecific organic anion transporter. J Bacteriol, 2000. 182(18): p. 5196-201.
59. Gilliland, S.E. and D.K. Walker, Factors to consider when selecting a culture of Lactobacillus acidophilus as a dietary adjunct to produce a hypocholesterolemic effect in humans. J Dairy Sci, 1990. 73(4): p. 905-11.
60. Chen, P.W., et al., Effects of bovine lactoferrin hydrolysate on the in vitro antimicrobial susceptibility of Escherichia coli strains isolated from baby pigs. Am J Vet Res, 2004. 65(2): p. 131-7.
61. Tian, H., et al., Influence of bovine lactoferrin on selected probiotic bacteria and intestinal pathogens. Biometals, 2010. 23(3): p. 593-6.